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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(9): 351-359
DOI: https://doi.org/10.20546/ijcmas.2020.909.045


Effect of Extender with or Without Centrifugation of Mongrel Dog Semen Preserved at Refrigeration Temperature (4°C)
Praveen Kumar1, Ankesh Kumar1, S. P. Singh1, J. K Prasad1,Sengupta1, S. K. Sheetal1* and Pallav Shekhar2
1Department of Veterinary Gynaecology and Obstetrics, 2Department of Veterinary Medicine, Bihar Veterinary College, Bihar Animal Sciences University, Patna, Bihar, India
*Corresponding author
Abstract:

In the present study, 3 mongrel dogs were taken for semen collection. Total of 18 ejaculates were collected, 6 from each dog by digital manipulation.  All the collected samples were randomly divided into three Groups viz. Group I (Non-extended semen), Group II (Semen extended with egg yolk tris citrate fructose extender) and Group III (Sperm pellet extended with egg yolk tris citrate fructose extender) and kept at refrigeration temperature (4oC). In Group III semen is centrifuged at 720 x g for 5 minutes by centrifuge machine Tarsons Spinwin MC05-R for making of sperm pellet. After centrifugation of semen, removed the supernatant from sample and the sperm pellet re-suspended in egg yolk tris citrate fructose extender at room temperature and re-diluted semen samples were placed at refrigeration temperature (4OC) for further semen evaluation on daily basis. The preserved semen were examined for individual progressive motility, live dead percent, sperm morphological abnormality, HOST (Hypo osmotic swelling test) and intact acrosome at each day of preservation till progressive motility goes down less than 40%. We found that motility goes down below 40% on day 2 in group I and on day 4 in group II and group III. Further sperm live dead percent, HOST (Hypo osmotic swelling test) and intact acrosome were higher in Group II as compared to Group I and III.


Keywords: Mongrel dog, Centrifugation, Prostatic fluid, Refrigeration temperature, Semen preservation
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How to cite this article:

Praveen Kumar, Ankesh Kumar, S. P. Singh, J. K Prasad, D. Sengupta, S. K. Sheetal and Pallav Shekhar. 2020. Effect of Extender with or Without Centrifugation of Mongrel Dog Semen Preserved at Refrigeration Temperature (4°C)Int.J.Curr.Microbiol.App.Sci. 9(9): 351-359. doi: https://doi.org/10.20546/ijcmas.2020.909.045