National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In the present study, 3 mongrel dogs were taken for semen collection. Total of 18 ejaculates were collected, 6 from each dog by digital manipulation. All the collected samples were randomly divided into three Groups viz. Group I (Non-extended semen), Group II (Semen extended with egg yolk tris citrate fructose extender) and Group III (Sperm pellet extended with egg yolk tris citrate fructose extender) and kept at refrigeration temperature (4oC). In Group III semen is centrifuged at 720 x g for 5 minutes by centrifuge machine Tarsons Spinwin MC05-R for making of sperm pellet. After centrifugation of semen, removed the supernatant from sample and the sperm pellet re-suspended in egg yolk tris citrate fructose extender at room temperature and re-diluted semen samples were placed at refrigeration temperature (4OC) for further semen evaluation on daily basis. The preserved semen were examined for individual progressive motility, live dead percent, sperm morphological abnormality, HOST (Hypo osmotic swelling test) and intact acrosome at each day of preservation till progressive motility goes down less than 40%. We found that motility goes down below 40% on day 2 in group I and on day 4 in group II and group III. Further sperm live dead percent, HOST (Hypo osmotic swelling test) and intact acrosome were higher in Group II as compared to Group I and III.
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