National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Plants of Cucurbitaceae family have shown to possess proteins like ribosome-inactivating proteins (RIPs) which have shown promising results against microbes and are also being explored for anti-cancer activities. The present study was designed to isolate a novel protein from a Cucucrbitaceae species which shows Ribosome inactivating property, and which in turn may be effective against cancer i.e. showing anticancer activity. Trichosanthes dioica seeds were taken which are not explored enough for proteins which can inactivate ribosomes and in turn may be used for their medicinal property in cancer treatment etc. Purification for Ribosome Inactivating Proteins (RIPs) resulted in an approx. 32KDa band from Trichosanthes dioica seeds. Ribosome inactivating property was tested using rabbit reticulocyte which gave a positive result. Anticancer activity of the isolated protein was tested on cancerous cell lines MDA and HL-60 along with PBLs as control cell line. The results showed that the protein is effective against cancerous cell lines with minimal effect on normal cell lines. The isolated protein was found to be less toxic to normal cells and more effective as anti-proliferative agents than Momordica charantia derived protein which was used as positive control. Also, the isolated protein showed a protein profile with predominance of alpha-helical elements. The study showed that N-glycosidase activity of the protein plays a great role as the mode of action as found in the secondary structure homology analysis.
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