National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The study was performed for development of macro and micro morphological characters of Aspergillus isolated from groundnut seeds, groundnut cake and soil using cultural and microscopoic methods. Fungus colonies was inoculated in different media like Potato Dextrose Agar (PDA) and Yeast Extract Sucrose (YES) Agar and compared with standard cultures. The Aspergillus isolates grown on both medium were identified using macroscopic characteristics such as colony color, colony reverse color, colony edge, mycelia color, conidation, shape of conidia, conidial wall, mycelium growth, mycelium growth rate and microscopic characteristics including vesicle diameter, conidiophore width, length of conidia head and ascospore diameter. According to colony colour, pigmentation and length of conidial head, the 21 isolates were identified as Aspergillus species viz., A. flavus (06) (200-800µm conidial head length), A. tamarii (05) (250-700 µm), A. flavus var. columnaris (05) (500-1200 µm), A. sojae (02) (300-900 µm), A. parasiticus (01) (350-900 µm), A. niveus (01) (250-350 µm) and A. fumigatus (01) (900-950 µm). This is the first report on identification and characterization of Aspergillus strains based on length of conidial head on PDA medium. Fungal toxicity was anticipated based on aflatoxin detection using biochemical test. Out of 21, 08 isolates were found to be afla-toxigenic based on biochemical test.
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