National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
White rust caused by a fungus Albugo candida, is a destructive disease of many economically important species of Brassica, to control this resistance breeding is important .Marker validation is useful for testing reported markers in determining the target phenotypes in autonomous populations and dissimilar genetic backgrounds and also involves testing the reliability of marker to predict the phenotype, which defines whether or not a marker could be used in routine screening for MAS. The current study was carried out with the objective to validating the already reported intron polymorphic marker (At 5g 41560 and At 5g 41940) from Arabidopsis at 1.2 cM from AcB1-A4.1. 102 RIL population derived from Varuna x Bio YSR of Brassica juncea were validated along with their parents. Phenotypic and genotypic data of RIL population confirmed At5g41560 marker was polymorphic to both the parents as well as differentiate the resistance and susceptible individual of the population and found to be validated. But At5g41940 was found to be not validated. The present study indicated that At5g41560 marker could be used for screening the resistance and susceptible genotypes in oilseed breeding programs. For the introgression of white rust resistance gene, at 5g 41560 marker could be used in MAS as it was not specific to genotype.
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