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Original Research Articles                      Volume : 9, Issue:7, July, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 4092-4100
DOI: https://doi.org/10.20546/ijcmas.2020.907.481


Nutritional and Epidemiological Requirements for Growth and Sporulation of Fusarium oxysporum f. sp. lentis caused Lentil Wilt
Meenu Kumari Meena*, C. B. Meena, D. L. Yadav, H. P. Meghwal, Sandhya and Karan Singh
Department of Plant Pathology, College of Agriculture, Ummedanj (Kota), India
*Corresponding author
Abstract:

Fusarium oxysporum f. sp. lentis was isolated from wilt infected lentil plant samples were collected from the field of AICRP on MULLaRP at Agriculture Research Station, Ummedganj, (Kota). In epidemiological studies, mycelium growth and sporulation by Fusarium oxysporum f. sp. lentis was significantly influenced with media, temperature and pH level. Maximum colony diameter (90.00 mm) recorded on oat meal agar (Hi-media) medium, which was statistically at par with potato dextrose agar (Hi-media) yielded 88.57 mm colony diameter, Both the media support excellently sporulation by pathogen. After 168 hrs of incubation, significantly higher mycelial growth (90.00 mm) was observed at 30°C, as compared to 25°C produced 87.00 mm colony diameter. A sudden fall in mycelial growth of Fusarium oxysporum f. sp. lentis was observed in both increasing and decreasing of temperature level. The excellent sporulation density was also observed under microscopic field at temperature 30°C and 25°C. It was found that pathogen grow at all pH levels tested but it was significantly higher at pH 6.0 (90.00 mm) 168 hrs. after incubation when compared with pH 5.0 (86.50 mm) and pH 7.0 (82.50 mm) found favourable pH level for mycelial growth, Excellent sporulation was observed at pH 6.0. Good sporulation was observed at pH 5.0 and 7.0.


Keywords: Lentil, Fusarium oxysporum f. sp. lentis, Culture media, pH, Temperature, Colony Growth and sporulation, lentil wilt

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How to cite this article:

Meenu Kumari Meena, C. B. Meena, D. L. Yadav, H. P. Meghwal, Sandhya and Karan Singh. 2020. Nutritional and Epidemiological Requirements for Growth and Sporulation of Fusarium oxysporum f. sp. lentis caused Lentil Wilt.Int.J.Curr.Microbiol.App.Sci. 9(7): 4092-4100. doi: https://doi.org/10.20546/ijcmas.2020.907.481
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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