International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:3, March, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(3): 2296-2299

Persistence of Antibiotic Residue in Milk under Region of Bihar, India
Ramesh Kumar Nirala*, Kumari Anjana, K.G. Mandal and C. Jayachandran
Department of Pharmacology and Toxicology, Bihar Veterinary College, Patna-800014, India
*Corresponding author

Indiscriminate use of antimicrobial drugs posses a great threat to human population. Their residues in milk over a long time may produce a variety of manifestations like individual drug toxicities including drug allergies, carcinogenicity and most importantly microbial resistance to these drugs. Keeping in view of the above facts, monitoring of residues of enrofloxacin and its metabolites were done in North Bihar region. With the objectives to estimate the residues of these antibiotics in milk samples, sample survey was done in organized as well as unorganized dairy sectors. A total number of 250 milk samples consisting of 125 from organized and 125 from unorganized sectors were randomly collected out of which 50 sample from each districts of Bihar. The samples were stored in deep freeze till analysis. Samples were processed before high performance liquid chromatography (HPLC) analysis as per standard analytical procedures. Analytical methods for estimation of residues of tetracyclines, sulfonamides and fluoroquinolones were standardized. The antimicrobial residues in milk were estimated above MRL values [MRL values in respect of tetracyclines, sulfonamides and fluoroquinolones is 0.1 µg/ml]. A total of five samples for tetracycline and two for oxytetracycline, two for sulfadimidine and one for sulfamethoxazole were found to contain residues above MRL values but no any sample of enrofloxacin and ciprofloxacin were found to concentration above MRL values i.e., 0.1 µg/ml. On examination of history of individual animal samples, it was found that animals were given either recent treatment with these antibacterials without observing proper withdrawal periods. Other sources may be feed additive as growth promoters.

Keywords: Antimicrobial, Milk, Cattle, Buffalo.

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How to cite this article:

Ramesh Kumar Nirala, Kumari Anjana, K.G. Mandal and Jayachandran, C. 2017. Persistence of Antibiotic Residue in Milk under Region of BiharInt.J.Curr.Microbiol.App.Sci. 6(3): 2296-2299. doi:
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