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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:7, July, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 3866-3879
DOI: https://doi.org/10.20546/ijcmas.2020.907.453


Isolation and Identification of Heterorhabditis bacteriophora from Tea Plantation of Assam
Bharath Amuri1*, Gitanjali Devi1, B. N. Choudhury1, P. Debnath2 and Diganta Bora3
1Department of Nematology,
2Department of Plant pathology,
3Department of Tea Husbandry and Technology, Assam Agricultural University Jorhat, Assam, India
*Corresponding author
Abstract:

A total 200 soil samples were collected from tea plantation areas of district, Jorhat, Assam and were assessed for entomopathogenic nematodes using the Galleria baiting technique. Out of 200 soil samples, EPN was found in 1 soil sample collected from Tea plantation, Chetiagaon, Jorhat and designated as EPN-H-1. Morphological and morphometrical characters were used for the identification of nematode isolate. The measurements were expressed in % ratios and Means ± SD ranges. The IJ of EPN-H-J-l showed close similarity with H. bacteriophora with respect to head shape, ratio b, D% and E%, but exhibited minor differences from the type measurements by having lower tail length (82 vs. 91). The males of this isolate showed close similarity with H. bacteriophora with respect to head shape, anal body width, gubernaculum length but exhibited minor differences from the type measurements by having higher esophagus length (107 vs. 103) and tail length (34 vs. 28). The hermaphroditic and amphimictic females of this isolate exhibited differences from the type measurements by having lower body length, lower body width and lower anal body width which are considered as intraspecific variations of H. Bacteriophora. Comparative analysis revealed that an EPN-H-1 isolate belong to the Heterorhabditis bacteriophora as earlier described by Poinar, 1976 from Brecon, South Australia in respect of body length, body width, tail length etc.


Keywords: Galleria, H. bacteriophora and Tea etc.

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How to cite this article:

Bharath Amuri, Gitanjali Devi, B. N. Choudhury, P. Debnath and Diganta Bora. 2020. Isolation and Identification of Heterorhabditis bacteriophora from Tea Plantation of Assam.Int.J.Curr.Microbiol.App.Sci. 9(7): 3866-3879. doi: https://doi.org/10.20546/ijcmas.2020.907.453
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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