National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Mastitis has been a major problem to the dairy industry and pose main impact on the economy of dairy farmers. Antibiotic resistance is increasing day by day and has become a big challenge worldwide with public health significance. In the present study antimicrobial resistance and sensitivity pattern of mastitis milk samples were assessed during the period from August, 2015 to March, 2020 at State level diagnostic laboratory, Sri Venkateswara Veterinary University, Tirupati. A total of 2,488 milk samples were subjected for cultural isolation and invitro antibiotic sensitivity test with standard disc diffusion method (Bauer et al., 1966). Out of 2,488 milk samples on cultural isolation, 2,174 (87.38%) of gram positive and 314 (12.62%) of gram negative bacteria were identified. Among 2,174 gram positive bacteria, Staphylococcus aureus -2,133(98.11%) was predominant followed by Streptococci-20 (0.92%) and bacillus species -19 (0.87%). Similarly, among 314 gram negative bacteria, E.coli-255(81.20%) were major pathogens followed by Klebsiella-53 (16.88%) and Pseudomonas -14 (4.46%) were identified. Invitro, antibiotic sensitivity test results of gram positive bacterial isolates (2,174) showed resistance and sensitivity to Amikacin (90.4% ; 9.60%) followed by Kanamycin (88.4%;11.6%), Streptomycin (87.5%; 12.50%), Tetracycline (79.6 ; 20.4%), Gentamycin (75.3% ; 24.7%), Pencillin (60.2% ; 39.8%),Ciprofloxacin (52.50 ; 45.7%), Enrofloxacin (48.9%; 51.20), Ampicillin (45.1%;54.9%) and Amoxycillin (36.4% ; 63.6%) respectively. Whereas the isolates of gram negative bacteria revealed resistance and sensitivity to Kanamycin-78.71%; 21.30% followed by Pencillin-76.81%; 23.10%, amikacin-69.11%; 30.90%), Tetracyclin (66.61% ; 33.40%), Streptomycin-(66.6%;33.40%), Ampicillin (64.70%;35.40), Gentamycin (51.0%; 49.0%), Ciprofloxacin (42.0% ; 58.0%) and Enrofloxacin-(34.71%;65.30%) (109/314).
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