International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:7, July, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 3089-3099

Effects of Ascorbic Acid as Antioxidant Semen Additive in Cryopreservation of Cross-bred Cattle Bull Semen
Padamveer Singh1, Sanjay Agarwal1*, Harjyote Singh1, Satbir Singh2, Pawan Kumar Verma3, Mohd Shaheem Buttand Utsav Sharma1
1Divison of Veterinary Gynaecology & Obstetrics, F.V.Sc. & A.H., S.K.U.A.S.T.-J., R. S. Pura-181102, Jammu, India
2Frozen Semen Laboratory, U.T. of Jammu & Kashmir, Hukkal, Jammu, India
3Division of Veterinary Pharmacology and Toxicology, F.V.Sc. & A.H., S.K.U.A.S.T.-J., R. S. Pura-181102, Jammu, India
*Corresponding author

The present study was jointly undertaken at the Frozen Semen Laboratory, U.T. of Jammu & Kashmir, Hukkal, Jammu and Division of V.G.O., F.V.Sc & A.H., SKUAST- J, R.S.Pura, during the period between December 2017 and May 2018. This investigation was carried out with the objective to study the effect of antioxidant semen additive ascorbic acid on cryopreservation of semen. Semen samples (n=10) from mature cattle bull stationed at Frozen semen laboratory, U.T. of Jammu & Kashmir, Hukkal, Jammu, were used to evaluate the effect of ascorbic acid additive at post-dilution and at post thaw stage. The semen sample was extended with Tris-Egg-Yolk-Citric-acid-Fructose-Glycerol (TEYCAFG) extender and were split into two groups: Group 1: TEYCAFG without any additive/ control and group 2: TEYCAFG + Ascorbic acid (5mM). Progressive motility, live spermatozoa, acrosomal integrity, sperm abnormality, hypo-osmotic swelling test (HOST) was evaluated at both post-dilution and post-thaw stage. Whereas, oxidative stress tests viz. malondialdehyde (MDA), catalase (CAT) and superoxide dismutase (SOD) were evaluated at only post-thawed stage. Group 2 i.e. ascorbic acid group, showed significant (p<0.05) increased live spermatozoa, acrosomal integrity and HOST positive spermatozoa, while significant (p<0.05) decreased sperm abnormalities in post-thawed semen. In oxidative stress evaluation the MDA level was significantly (p<0.05) decreased, whereas, SOD levels significantly (p<0.05) increased in group 2 in comparison to control group. It was concluded that addition of ascorbic acid (5 mM) as semen additive improves semen quality and minimize oxidative stress to the spermatozoa during cryopreservation of semen.

Keywords: Semen, Cattle bull, Ascorbic acid, Oxidative stress

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How to cite this article:

Padamveer Singh, Sanjay Agarwal, Harjyote Singh, Satbir Singh, Pawan Kumar Verma, Mohd Shaheem Butt and Utsav Sharma. 2020. Effects of Ascorbic Acid as Antioxidant Semen Additive in Cryopreservation of Cross-bred Cattle Bull Semen.Int.J.Curr.Microbiol.App.Sci. 9(7): 3089-3099. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.