Pointed gourd (Trichosanthes dioica Roxb.) is one of the important nutritive cucurbit perennial vegetables in India and other tropical countries. In the present investigation, an efficient and reproducible protocol for clonal multiplication through in vitro culture of Pointed gourd (Trichosanthes dioica Roxb.) is standardized. High frequency organogenic callusing from shoot-tip and nodal segments was induced by culturing the explants on MS medium supplemented with 4µM 2-4-D. The maximum regeneration of shoots from callus (72.47 %) and highest callus weight per explant (0.5 g) was recorded in variety ‘Nayagarh Local (Female)’. The highest number of shoots (3.0) per callus was regenerated from variety ‘Swarna Aloukik (Female)’ when cultured in MS medium fortified with BAP (4 µM). The rooted plantlets were transferred to soil mixture (soil: sand: cocopit; 1:1:1) and kept in greenhouse with 85% humidity. After 2-3 weeks hardening, the plantlets were successfully transplanted in the polybag or in the field with higher survival rate (85%). No detectable variation in the regenerated plants with respect to morphology or growth characteristics was detected in the field as compared to the mother plant.These results may be great helpful for production of number of quality, uniform, disease free and desired plantlets for distribution to the farmers.

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