International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:7, July, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 2252-2258

Effect of Prostatic Fluid and Different Cryoprotectants on post-thaw Dog Spermatozoa
Kaushal Kusum1*, Harendra Kumar Gupta2, Ramakrishna Roy3, Raghavendra Prasad Mishraand Saurabh5
1Department of Veterinary Gynaecology and Obstetrics, Apollo College of Veterinary Medicine, Jaipur, India
2Division of Animal Reproduction, ICAR-Indian Veterinary Research Institute (IVRI), Izzatnagar, Bareilly-243122, India
3Krishi Vigyan Kendra, Sipaya, Gopalganj, Bihar (Dr. Rajendra Prasad Central Agricultural University), India
4Department of Veterinary Public Health,
5Department of Livestock Farm Complex, Apollo College of Veterinary Medicine, Jaipur, India
*Corresponding author

Seminal plasma in dogs is prostatic in origin. Prostatic fluid plays role in immunological, motility and energy supply. Forty eight ejaculates of sperm rich fraction were collected from four adult male spitz dogs. Prostatic fluid was collected from the third fraction. Sperm rich semen was diluted (1:2) with Tris Fructose Egg Yolk Glycerol and divided into four cryoprotectant groups viz 8 % Glycerol (Group I, control), 4 % Glycerol (Group II), 5 % Ethylene Glycol (Group III) and 4 % Glycerol+4% Ethylene Glycol (Group IV), cryopreserved for 24 hrs thereafter thawed and prostatic fluid added with the objective to study motility, viability, abnormality, Hypo-osmotic swelling test (HOSST) and acrosomal integrity. Addition of prostatic fluid post thaw significantly increased motility compared to no addition of prostatic fluid in all cryoprotectants however other parameters such as livability, abnormality, HOSST and acrosomal integrity remained changed. Among the post thaw prostatic fluid added groups, Group III ( 5 % Ethylene glycol) had significantly higher (P<0.01) motility, livability, Hypo-osmotic swelling Test (HOSST) and acrosomal integrity whereas significantly lower (P<0.01)in Group IV, abnormality as compared to Group I, control (8 % Glycerol). The other cryoprotectants Group II (4 % Glycerol) and Group III (4 % Glycerol + 4 % Ethylene Glycol) performed higher in Group IV.

Keywords: Dog, Prostatic fluid, Cryoprotectants, Post-thaw, Motility, Viability, HOSST, Acrosomal integrity

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Kaushal Kusum, Harendra Kumar Gupta, Ramakrishna Roy, Raghavendra Prasad Mishra and Saurabh. 2020. Effect of Prostatic Fluid and Different Cryoprotectants on post-thaw Dog SpermatozoaInt.J.Curr.Microbiol.App.Sci. 9(7): 2252-2258. doi:
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