National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Seminal plasma in dogs is prostatic in origin. Prostatic fluid plays role in immunological, motility and energy supply. Forty eight ejaculates of sperm rich fraction were collected from four adult male spitz dogs. Prostatic fluid was collected from the third fraction. Sperm rich semen was diluted (1:2) with Tris Fructose Egg Yolk Glycerol and divided into four cryoprotectant groups viz 8 % Glycerol (Group I, control), 4 % Glycerol (Group II), 5 % Ethylene Glycol (Group III) and 4 % Glycerol+4% Ethylene Glycol (Group IV), cryopreserved for 24 hrs thereafter thawed and prostatic fluid added with the objective to study motility, viability, abnormality, Hypo-osmotic swelling test (HOSST) and acrosomal integrity. Addition of prostatic fluid post thaw significantly increased motility compared to no addition of prostatic fluid in all cryoprotectants however other parameters such as livability, abnormality, HOSST and acrosomal integrity remained changed. Among the post thaw prostatic fluid added groups, Group III ( 5 % Ethylene glycol) had significantly higher (P<0.01) motility, livability, Hypo-osmotic swelling Test (HOSST) and acrosomal integrity whereas significantly lower (P<0.01)in Group IV, abnormality as compared to Group I, control (8 % Glycerol). The other cryoprotectants Group II (4 % Glycerol) and Group III (4 % Glycerol + 4 % Ethylene Glycol) performed higher in Group IV.
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