National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Survey of Sathgudi sweet orange was done in the Nagri village of Chittor district in the A.P. state during November, 2006. Incidence of citrus yellow mosaic disease was observed up to 50% in citrus plants. A few plants also showed unusual symptoms of vein clearing and mosaic. Bacilliform and flexous rod shaped of virus particles were seen in electron microscopy in samples of the diseased citrus plants showing vein clearing and mosaic. Immunosorbent electron microscopy indicated the baciliform particles were of Citrus Yellow Mosaic Virus (CYMV) while flexuous particles were of Indian citrus ringspot virus. Since ISEM can not be used for routine detection, a duplex PCR for detection of both the viruses was standardized, using specific primers of CYMV and ICRSV providing amplification product of 537 bp and 1121bp. The sequencing of PCR product confirmed that the amplified PCR product of ICRSV and CYMV. The duplex PCR provide a useful and rapid method for molecular detection of two viruses. The technique should prove highly useful in disease surveys, nursery certification and quarantine applications.
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