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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:7, July, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 645-656
DOI: https://doi.org/10.20546/ijcmas.2020.907.074


Nutritional and Epidemiological Requirements for Growth and Sclerotia Formation by Sclerotium rolfsii (Sacc.) Causing Collar Rot of Chickpea (Cicer areitinum L.)
Karan Singh*, C. B. Meena, Chirag Gautam, B. K. Patidar and N. R. Koli
Department of Plant Pathology, College of Agriculture, Ummedanj (Kota), India
*Corresponding author
Abstract:

Sclerotium rolfsii was isolated from infected chickpea plant sample collected from the chickpea’s field of Agriculture Research Station, Ummedganj, (Kota). In epidemiological studies, mycelium growth and sclerotia formation by S. rolfsii was significantly influenced with media, temperature and pH level. Maximum colony diameter (90.00 mm) and sclerotia count (691.00 per plate) were recorded on oat meal agar (Hi-media) at 96 hrs. and 20 days after inoculation respectively, followed by potato dextrose agar (natural) in radial mycelial growth 87.00 mm and potato dextrose agar (Hi-Media) in number of sclerotia count 665.00 per plate. Maximum colony diameter (90.00 mm) and sclerotia counts (538.50) of the pathogen were recorded at the 25 ºC temperature was statistically superior over rest temperature level tried. While, in context to pH maximum colony diameter 89.00 mm of pathogen and sclerotia count 570.00 sclerotia per plate were recorded at the pH level 7.0.


Keywords: Chickpea, Sclerotium rolfsii, Culture media, pH, Temperature, Colony Growth and Sclerotia formation

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How to cite this article:

Karan Singh, C. B. Meena, Chirag Gautam, B. K. Patidar and Koli. N. R. 2020. Nutritional and Epidemiological Requirements for Growth and Sclerotia Formation by Sclerotium rolfsii (Sacc.) Causing Collar Rot of Chickpea (Cicer areitinum L.).Int.J.Curr.Microbiol.App.Sci. 9(7): 645-656. doi: https://doi.org/10.20546/ijcmas.2020.907.074
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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