National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Leptin gene could be considered as a hotspot gene and its role in meat and milk production makes it a candidate for the search of molecular markers related to meat production as well as meat quality in goats. The present investigation was carried out to study the occurrence of polymorphism in Leptin (LEP) gene in Assam Hill Goat and to associate the polymorphic gene, if any, with body weights of the goats at 0 day, 3, 6, 9 and 12 months of age. A total of 256 blood samples were collected from male goats maintained at different field units and at the Goat Research Station, Assam Agricultural University, Burnihat under the “ICAR- All India Coordinated Research Project on Goat Improvement”. PCR-RFLP was analysed with CViQI restriction enzyme with restriction site as GË„TAC. Multiple alignment of the sequences of the representative amplicons revealed no nucleotide mutation at the restriction site. All the 256 samples produced two fragments of 124 bp and 400 bp in 12% Polyacrylamide Gel Electrophoresis (PAGE) revealing monomorphic banding patterns. As no polymorphism could be established, further study on the association of polymorphic genotypes with the body weights at different ages could not be carried out.
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