International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:7, July, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 374-379

Etiology and Antibiogram of Subclinical Mastitis in Cows of Puri District, India
P. Sahoo, N. Sahoo* and S. Biswal
Department of Epidemiology and Preventive Medicine, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneswar -751003, India
*Corresponding author

Subclinical mastitis (SCM), predominantly caused by Gram positive bacteria, continues to be a global problem of dairy cows including India with negative impact on economic growth as well as public health. SCM was detected in 283 cross-bred Jersey milch cows between 2nd to 5th lactation from a milk‒shed village of Odisha on the basis of cow‒side test (Modified California Mastitis Test) and cultural examination. Polymerase chain reaction using universal 16S primers followed by Sangers sequencing helped to identify Staphylococcus aureus in maximum (49.8%) cows followed by Streptococcus uberis (14.8%), Streptococcus agalactiae (8.1%), Streptococcus dysagalactiae (7.8%) and Streptococcus pyogenes (3.8%), Escherichia coli (2.1 %), Proteus sp (1.8 %) and Klebsiella pneumonia (1.4 %). In vitro antibiotic sensitivity test was performed by disc diffusion method. Anitibiotics showing sensitivity to more than 90 per cent of milk samples were ceftiofur (98.3%), cefuroxime (97.34%), gentamicin (95.52%), ceftizoxime (94.09%), cephalexin (92.47%) and cefoperazone (90.91%).

Keywords: Gram positive bacteria, dairy cows, In vitro

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How to cite this article:

Sahoo. P., N. Sahoo and Biswal. S. 2020. Etiology and Antibiogram of Subclinical Mastitis in Cows of Puri District, India.Int.J.Curr.Microbiol.App.Sci. 9(7): 374-379. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.