National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Subclinical mastitis (SCM), predominantly caused by Gram positive bacteria, continues to be a global problem of dairy cows including India with negative impact on economic growth as well as public health. SCM was detected in 283 cross-bred Jersey milch cows between 2nd to 5th lactation from a milk‒shed village of Odisha on the basis of cow‒side test (Modified California Mastitis Test) and cultural examination. Polymerase chain reaction using universal 16S primers followed by Sangers sequencing helped to identify Staphylococcus aureus in maximum (49.8%) cows followed by Streptococcus uberis (14.8%), Streptococcus agalactiae (8.1%), Streptococcus dysagalactiae (7.8%) and Streptococcus pyogenes (3.8%), Escherichia coli (2.1 %), Proteus sp (1.8 %) and Klebsiella pneumonia (1.4 %). In vitro antibiotic sensitivity test was performed by disc diffusion method. Anitibiotics showing sensitivity to more than 90 per cent of milk samples were ceftiofur (98.3%), cefuroxime (97.34%), gentamicin (95.52%), ceftizoxime (94.09%), cephalexin (92.47%) and cefoperazone (90.91%).
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