An investigation on the project entitled “Effect of bio-fertilizers on growth behaviour and quality parameters of garlic (Allium sativum Linn.)” was taken up in the horticultural garden of Janta College, Bakewar, Etawah, U.P. which falls under sub-tropical climatic region during 2011-12 to work out the optimum dose of the three bio-fertilizer and their combination on garlic variety-G1. Fourteen treatments namely, a control receiving FYM as basal dose @ 20 ton/ha + RDF (100:50:50) (T0), Azotobacter 6 kg/ha (T1), Azotobacter 8 kg/ha (T2), PSB 6 kg/ha (T3), PSB 8 kg/ha (T4), VAM 6 kg/ha (T5), VAM 8 kg/ha (T6), Azotobacter 6 kg/ha+ PSB 6 kg/ha+ VAM 6 kg/ha (T7), Azotobacter 6 kg/ha+ PSB 8 kg/ha+ VAM 8 kg/ha (T8), Azotobacter 6 kg/ha+ PSB 6 kg/ha+ VAM 8 kg/ha (T9), Azotobacter 6 kg/ha+ PSB 8 kg/ha+ VAM 6 kg/ha (T10), Azotobacter 8 kg/ha+ PSB 6 kg/ha+ VAM 6 kg/ha (T11), Azotobacter 8 kg/ha+ PSB 8 kg/ha+ VAM 8 kg/ha (T12), Azotobacter 8 kg/ha+ PSB 6 kg/ha+ VAM 8 kg/ha (T13), Azotobacter 8 kg/ha+ PSB 8 kg/ha+ VAM 6 kg/ha (T14) applied into soil prior to transplanting of seed cloves. The experiment was carried out in Randomized Block Design with 3 replications having plot size 3 sq.mt. The combination of treatments namely Azotobacter 8 kg/ha+ PSB 8 kg/ha+ VAM 8 kg/ha (T12) resulted the best optimal doze for garlic givingplant height 79.83 cm, average number of leaves 9.86, average diameter of pseudo stem 1.13 cm, average volume of bulb 48.23 cc and T12 produced 117.67 numbers of ‘A’ grade bulbs or 67.24% out of 175 bulbs.

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