National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The aim of the study was to evaluate and compare Boer buck sperm motility with 5 mM Glutathione (G) and or 1% or 2% Honey (H) in Skim milk (SM) based extenders preserved at refrigeration temperature at 0, 24, 48 and 72 hrs. A total of 72 ejaculates were collected equally from 6 mature bucks at weekly interval by using Artificial Vagina (AV) as per standard procedure. All the ejaculates were diluted using six Skim milk based extenders viz. SME, SMGE, SMGH(1%)E, SMGH(2%)E, SMH(1%)E and SMH(2%)E. The sperm motility was evaluated by CASA (Computer Assisted Semen Analyzer). The data obtained was statistically analyzed by General Linear Model (GLM) and procedure. The results showed that sperm motility was differed significantly (P<0.05) from extender to extender at 24 h, 48 h and 72 h of refrigeration. Based on the results concluded that supplementation of optimum concentration of glutathione (5 mM) and honey (1%) maintained better sperm motility upto 72 hours of storage and successful preservation of buck spermatozoa at refrigeration temperature. Hence study revealed that Boer buck semen can be preserved effectively with SMGH(1%)E, at refrigeration temperature upto 72 hours of storage.
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