National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was aimed to prepare a conjugate of Fc fragment of mice IgG with rOMP 28 of Brucella melitensis and investigate its ability to influence the kinetics of humoral and cellular immune response in comparison to its unconjugated counterpart and the rOMP 28 alone in the mice model. Among all the groups, the group of mice immunized with rOmp28 alone induced highest level of humoral immune response and the vaccine strain group(S19) conferred highest level of cellular response which was evidenced by higher nitric oxide concentration and the clearance of virulent B. abortus 544 from the spleen upon challenge infection. The conjugated group conferred low level of humoral response compared to the rOMP28 alone which may be due to the involvement of inhibitory FcγIIR in the regulation of immune responses, soluble forms of Fcï§R inhibiting antigen presentation and irreversible loss of Fc receptors on the macrophage plasma membrane. The S19 strain of B. abortus, a live vaccine conferred high level of protection when challenged with virulent B. abortus 544which indicate whole cell organism composed of various immunogenic components might have been involved in inducing protection.
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