National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
During survey, tomato leaf samples showing leaf curl symptoms were collected from different tomato fields of Dharwad, Haveri and Kolar districts. Total nucleic acid extraction was carried out by cetyl trimethyl ammonium bromide (CTAB) method and quantified to perform polymerase chain reaction using Begomovirus group specific coat protein primers targeting the coat protein region and were sequenced. The nucleotide sequences were analyzed using the BLAST tool for which results indicated the presence of Tomato leaf curl virus (ToLCV), Croton yellow vein mosaic virus (CYVMV) and Chilli leaf curl virus (ChiLCV) that shows the association of these viruses in leaf curl symptoms in tomato obtained from the fields. The cluster analysis for all the ten sequences showed the grouping of the Chilli leaf curl virus and Tomato leaf curl virus into separate groups. Besides, the CYVMV was sub-grouped in ToLCV group. Thus this study shows the occurrence of different Begomoirus in leaf curl symptoms associated tomato growing in different regions of Karnataka.
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