National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Trichomonas vaginalis is an important cause of the premature rupture of membranes, premature delivery, pelvic inflammatory disease, urethritis, and chronic prostatitis. Wet mount is the most widely used method for the detection of T. vaginalis in clinically suspected cases of vulvovaginitis. The purpose of this Study was to compare various microscopic techniques for detection of T. vaginalis like wet mount examination, Giemsa and Acridine orange staining. The Cross-sectional Analytical study was conducted in the Department of Microbiology, attached to a Tertiary Care Hospital in Central India after obtaining ethical clearance over a time period of two months. Out of 30 cases, only two (6.66%) were detected positive for Trichomoniasis. All the 30 vaginal samples (cases) tested for detection of T. vaginalis were found to be negative by Wet mount examination. Whereas, Giemsa staining and Acridine orange staining were positive in 1 (3.33%) and 2 (6.66%) case respectively. Present study highlights that Acridine orange staining was found to be the best microscopic method when compared with wet mount examination and Giemsa staining. This will also provide rapid screening of vaginal smears in patients suffering from vaginal discharge and thus, help in early diagnosis and prompt treatment of patients.
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