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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(3): 2883-2893
DOI: https://doi.org/10.20546/ijcmas.2020.903.332


DNA Sequence Variation within STAT1 Gene of Gaolao Cattle
P. M. Khandare1, D. S. Kale1*, P. G. Koringa2, D. V. Patil1 and A. P. Dhok3
1Department of Animal Genetics and Breeding, Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University (MAFSU), Nagpur, India
2Department of Animal Biotechnology, College of Veterinary Science and A.H., Anand Agricultural University, Anand, India
3Department of Animal Nutrition, Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University (MAFSU), Nagpur, India
*Corresponding author
Abstract:

The current research was designed to study DNA sequence variation within Signal transducer and activator of trancription 1 (STAT1) gene and to find association of STAT1 gene variants (if any) with milk traits in Gaolao cattle. DNA was isolated from around 40 Gaolao cows from farmers herd and farm in breeding tract. The STAT1 gene regions of 314 bp, 850 bp and 154 bp fragments were amplified using STAT1G1, STAT1G2 and STAT1G4 primers respectively. PCR-RFLP analysis of 314 bp 3’ UTR fragment of STAT1 gene using PagI restriction enzyme, revealed the CC genotype in Gaolao cattle indicating monomorphic status of the locus. The alignment analysis of 222 bp STAT1G1 sequence revealed SNP T-C at 221st position as polymorphic site. STAT1G2 primer amplified 783 bp sequence which revealed 03 polymorphic sites as SNP G-T at 724th position, SNP A-G at 730th position and SNP C-G at 736th position. The polymorphic sites were identified in STAT1 gene sequence indicating sequence variation in the STAT1 gene regions in Gaolao Cattle. The identified polymorphic sites may be validated with bigger phenotypic dataset and the results of which may prove significant for identification of markers related with milk production traits.


Keywords: Gaolao Cattle, PCR-RFLP, STAT1, SNP, Sequencing
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How to cite this article:

Khandare. P. M., D. S. Kale, P. G. Koringa, D. V. Patil and Dhok. A. P. 2020. DNA Sequence Variation within STAT1 Gene of Gaolao Cattle.Int.J.Curr.Microbiol.App.Sci. 9(3): 2883-2893. doi: https://doi.org/10.20546/ijcmas.2020.903.332