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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(3): 2256-2264
DOI: https://doi.org/10.20546/ijcmas.2020.903.256


Estimation of Quality Parameters, Antagonistic Properties and Commercial Production of Pseudomonas fluorescens in Plant Disease Management
Aradhana Mishra1, Vibha Agrawal 1, Ekta Kumari2 and Virendra Kumar3*
1Department of Biotechnology, Shree Agrasen Kanya P.G. College, Varanasi, U.P., India
2Central Insecticides Board & Registration Committee, Directorate of Plant Protection Quarantine and Storage, NH-IV, Faridabad-121 001, Haryana, India
3Bioassay Division, Central Insecticides Laboratory, Directorate of Plant Protection Quarantine and Storage, NH-IV, Faridabad-121 001, Haryana, India
*Corresponding author
Abstract:

Pseudomonas fluorescens is an aerobic, gram-negative, ubiquitous bacterium present in agricultural soils that is well adapted to grow in the rhizospheric as well as phyllospheric zone. An experiment was conducted at Shree Agrasen Kanya P.G. College, Varanasi, U.P. India aimed to determine the morphological features of P. fluorescens as well as to carry out the process and method of its commercial production of powder formulation of the bio-control agent P. fluorescens 1 % W.P for its effective use at field. Its morphology is characterized visually and microscopically by colony growth such as colony form, color, edge and Gram staining at laboratory. The quality parameters of P. fluorescens were checked for pH, moisture content, cfu, suspensibility and antagonistic capability. In this paper the detail protocol have been mentioned for preparation of talc base powder formulation of P. fluorescens and how to check various quality parameters. P. fluorescens was found most effective with highest antagonistic activity against six chosen fungal pathogen that showed maximum inhibition of mycelia growth of Fusarium oxysporum (62.60 %), Aspergillus niger (55.47 %), Rhizoctonia solani (53.8 %), Macrophomina phaseolina (52.30%), Alternaria alternata (49.58 %) and Pyricularia oryzae (47.67%).


Keywords: Pseudomonas fluorescens, pH, contaminants, suspensibility, antagonistic capability, Bio-pesticide
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How to cite this article:

Aradhana Mishra, Vibha Agrawal, Ekta Kumari and Virendra Kumar. 2020. Estimation of Quality Parameters, Antagonistic Properties & Commercial Production of Pseudomonas fluorescens in Plant Disease Management.Int.J.Curr.Microbiol.App.Sci. 9(3): 2256-2264. doi: https://doi.org/10.20546/ijcmas.2020.903.256