International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:3, March, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(3): 1792-1803

Influence of Plant Growth Regulators on Micropropagation of Gwarpatha [Aloe vera (L.) Burm.]
Mohan Lal Jakhar*, Divya Dixit, Sarfraz Ahmad, Mahaveer Prasad Ola, Manohar Ram and Hari Ram Jat
Department of Plant Breeding and Genetics, S.K.N. Agriculture University, Jobner, Rajasthan-303029, India
*Corresponding author

Present investigation was carried out to determine the optimum level of different plant growth regulators for rapid shoot multiplication, rooting and hardening of micropropagated plantlets of Aloe vera. The experiment was laid out using completely randomized design (CRD) with ten replications during 2018-19. Auxiliary shoot explants were inoculated on MS medium supplemented with various concentrations of cytokinin (BAP 0.5 – 6.0 mg/l) alone and BAP (0.5-6.0 mg/l) with auxin (NAA 0.1 – 0.6) in combination for direct shoot bud proliferation. For rooting, auxins (NAA/IBA) were used at the concentrations of 0.5-2.0 mg/l singly and 0.5-2.0 mg/l NAA with IBA at 0.5 and 2.0 mg/l in combinations. Maximum shoot bud induction was obtained when MS medium supplemented with 4.0 mg/l BAP singly and 4.5 mg/l BAP with 0.6 mg/l NAA in combination. Maximum rooting was induced at 1.5 mg/l IBA and 0.5 mg/l IBA with 2.0 mg/l NAA in combination. The rooted plantlets showed 80 per cent survival in culture room and 74 per cent in shade house during the process of hardening. Further, it is recommended that the given protocols may be used for mass multiplication of Aloe vera to meet the demand of farmers as well as pharmaceutical industries.

Keywords: Aloe vera, Gwarpatha, Micropropagation, Plant growth regulator, Root induction, Shoot proliferation, Hardening.

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How to cite this article:

Mohan Lal Jakhar, Divya Dixit, Sarfraz Ahmad, Mahaveer Prasad Ola, Manohar Ram and Hari Ram Jat. 2020. Influence of Plant Growth Regulators on Micropropagation of Gwarpatha [Aloe vera (L.) Burm.].Int.J.Curr.Microbiol.App.Sci. 9(3): 1792-1803. doi:
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