National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Present investigation was carried out to determine the optimum level of different plant growth regulators for rapid shoot multiplication, rooting and hardening of micropropagated plantlets of Aloe vera. The experiment was laid out using completely randomized design (CRD) with ten replications during 2018-19. Auxiliary shoot explants were inoculated on MS medium supplemented with various concentrations of cytokinin (BAP 0.5 – 6.0 mg/l) alone and BAP (0.5-6.0 mg/l) with auxin (NAA 0.1 – 0.6) in combination for direct shoot bud proliferation. For rooting, auxins (NAA/IBA) were used at the concentrations of 0.5-2.0 mg/l singly and 0.5-2.0 mg/l NAA with IBA at 0.5 and 2.0 mg/l in combinations. Maximum shoot bud induction was obtained when MS medium supplemented with 4.0 mg/l BAP singly and 4.5 mg/l BAP with 0.6 mg/l NAA in combination. Maximum rooting was induced at 1.5 mg/l IBA and 0.5 mg/l IBA with 2.0 mg/l NAA in combination. The rooted plantlets showed 80 per cent survival in culture room and 74 per cent in shade house during the process of hardening. Further, it is recommended that the given protocols may be used for mass multiplication of Aloe vera to meet the demand of farmers as well as pharmaceutical industries.
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