Trichoderma isolates were collected from the rhizosphere and rhizoplane of Chickpea and Rice ranged from 5^°C to 45^°C screened for temperature tolerance. Among all the temperature tested at 30^°C the isolates showed maximum average mycelial diameter of 8.81cm. Minimum average colony diameter of 0.87 cm was recorded at 40⁰C. Simultaneously isolates Ta12 showed maximum average colony diameter of 6.36 cm, which did not differ significantly from Ta14 (6.30 cm) and Ta13 (6.16 cm). It was interesting to note that Isolate Ta15 showed maximum colony diameter (2.60 cm) at 40⁰C, while most of the other isolates were not able to grow at this temperature. Similarly maximum (266.06 mg) average mycelial fresh weight of all the isolates were recorded at 30^°C followed by 20^°C (229.42 mg). Significantly maximum (143.50 mg) fresh weight was recorded at 40^°C by Isolate Ta15. Clearly 30⁰C is the best temperature for Trichoderma to grow and reproduce efficiently using as biocontrol agent.

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