International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:3, March, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(3): 1164-1171

Isolation and Evaluation of Temperature Tolerant Trichoderma
Manju Sharma*, Stanzin Idong, Roopali Sharma and Priya Singh
Department of Plant Pathology, GB Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India
*Corresponding author

Trichoderma isolates were collected from the rhizosphere and rhizoplane of Chickpea and Rice ranged from 5°C to 45°C screened for temperature tolerance. Among all the temperature tested at 30°C the isolates showed maximum average mycelial diameter of 8.81cm. Minimum average colony diameter of 0.87 cm was recorded at 400C. Simultaneously isolates Ta12 showed maximum average colony diameter of 6.36 cm, which did not differ significantly from Ta14 (6.30 cm) and Ta13 (6.16 cm). It was interesting to note that Isolate Ta15 showed maximum colony diameter (2.60 cm) at 400C, while most of the other isolates were not able to grow at this temperature. Similarly maximum (266.06 mg) average mycelial fresh weight of all the isolates were recorded at 30°C followed by 20°C (229.42 mg). Significantly maximum (143.50 mg) fresh weight was recorded at 40°C by Isolate Ta15. Clearly 300C is the best temperature for Trichoderma to grow and reproduce efficiently using as biocontrol agent.

Keywords: Biocontrol agent, Trichoderma, Isolation, Evaluation and Crop health management

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How to cite this article:

Manju Sharma, Stanzin Idong, Roopali Sharma and Priya Singh. 2020. Isolation and Evaluation of Temperature Tolerant Trichoderma.Int.J.Curr.Microbiol.App.Sci. 9(3): 1164-1171. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.