National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Litchi is considered a crop difficult to propagate through micropropagation and obtaining contamination free cultures is first and foremost requirement of tissue culture. So the present investigation was carried out to standardize the sterilization procedure of nodal segment and leaf explants of Litchi chinensis Sonn. cv. Purbi. Two surface sterilizing agents viz. HgCl2 and NaOCl were used at varying concentrations and durations. In this experiment HgCl2 was found to be better sterilizing agent than NaOCl for both the explants. HgCl2 (0.2 %) for 3 min treatment was found to be the most effective for nodal segment explants that resulted in maximum per cent survival (56.5±1.19%), low percent mortality (13.8±0.18%) and low per cent contamination (33.1±0.51%). While for leaf explants HgCl2 (0.1 %) for 1.0 min yielded best results with highest per cent survival (53.9±1.08), low per cent mortality (14.0±0.21) and low per cent contamination (35.5±0.56).
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