National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present investigation was carried out at Tissue Culture Laboratory of S.K.N. Agriculture University, Jobner, India during 2018-19. Experiment was laid out using completely randomized design (CRD) with ten replications. Six culture media viz. Murashige and Skoog media, Nitsch & Nitsch media, woody plant medium, Schenk and Hildebrant medium, White’s medium and Khudson solution–C were tested for direct shoot proliferation and callus induction at most responsive level of plant growth regulators in Bael (Aegle marmelos L.). Highest shoot bud induction (4.1) was observed using woody plant medium with 100 per cent morphogenetic response using nodal segment explant along with BAP at 2 mg/l concentration. Maximum callus induction on leaf explant was observed on Murashige and Skoog medium with 40 per cent morphogenetic response followed by woody plant medium (30 per cent) with 2,4-D at 2 mg/l concentration. Thus for large scale micropropagation, woody plant medium using nodal segment explant is recommended for shoot proliferation and for callus induction, MS medium taking leaf as explant will be rewarding for mass multiplication of Bael.
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