National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Opportunistic infections are infections that occur due to a decrease in the immune system and can cause serious conditions in people with weak immune systems such as in people with HIV / AIDS. Cryptosporidium sp. and Giardia lamblia are examples of the opportunistic intestinal protozoa that can cause chronic diarrhea so that it becomes dehydrated and malnutrition which can increase mortality and morbidity in HIV sufferers. Observational study with cross-sectional design on 50 fecal samples of HIV / AIDS patients. Cryptosporidium sp. and Giardia lamblia infections tested microscopically using Ziehl Neelsen's staining and molecular examination with DNA isolation,and looking at the relationship between CD4 cell counts and history of diarrhea with Cryptosporidium sp.and Giardia lamblia infections. The results obtained 2 respondents (4%) positive infected with Cryptosporidium sp., There is a significant relationship between CD4 cell counts with Cryptosporidium sp. infection, p-value=0.001 (p<0.05) and there is a significant relationship between Cryptosporidium sp.infections with a history of diarrhea, p-value=0.045 (p<0.05). And obtained 1 respondents (2%) positive infected with Giardia lamblia, There is no significant relationship between CD4 cell counts with Giardia lamblia. infection, p-value=1.000 (p>0.05) and there is a significant relationship between Giardia lamblia infections with a history of diarrhea, p-value=0.031 (p<0.05).18 out of 50 samples have been successfully carried out the process of isolating DNA specifically thick cell walled microorganisms.
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