National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Horse gram leaf samples showing yellow mosaic symptoms gave positive results with HgYMV specific primer pairs (HgYMV-CP-F/HgYMV-CP-R) and yielded amplicons of ~1000 bp whereas no PCR product was obtained from DNA extracted from healthy samples in PCR. The 1000 bp PCR products were cloned, sequenced, assembled and its length was determined as 885 bp including 111 bp pre-coat protein at 5’ end and 774 core coat protein. The length of the predicted amino acid sequence was found to be 257. Phylogenetic tree based on full length coat protein gene sequences of HgYMV with 23 other geminivirus sequences downloaded from NCBI Genbank formed three major clusters of MYMIV, HgYMV and MYMV. The present isolate formed unique cluster with HgYMV group that cause yellow mosaic disease symptoms in horsegram (AJ627904.1) and frenchbean (GU323321.1). Comparison of complete nucleotide sequence of coat protein gene of HgYMV indicated that coat protein gene of HgYMV-GKVK-Bangalore isolate had maximum homology with HgYMV-Tamil Nadu:HG [AJ627904.1] (99.2%), followed by HgYMV-Srilanka:FB [GU323321.1] (95.2%). HgYMV-GKVK-Bangalore isolate had 85.2-86.9 per cent identity and 80.2-81.9 per cent identity with MYMV and MYMIV isolates, respectively. Comparison of predicted amino acid sequence of coat protein gene of HgYMV revealed that HgYMV-GKVK-Bangalore isolate shared maximum amino acid identity with HgYMV-Tamil Nadu:HG [AJ627904.1] (99.2%), followed by HgYMV-Srilanka:FB [GU323321.1] (97.2%). HgYMV-GKVK-Bangalore isolate had 91-92.2 per cent identity and 82.8-84.8 per cent identity with MYMV and MYMIV isolates, respectively. From the above results, it is clear that HgYMV-GKVK-Bangalore is closely related to HgYMV isolates rather than MYMV and MYMIV.
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