International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:2, February, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(2): 1279-1286

Isolation, Characterization and Antibacterial Susceptibility test of Acinetobacter Species Obtained from Tertiary Care Hospital
Preeti Chandra1, Vineeta Mittal2 and Rachna Chaturvedi1*
1Amity Institute of Biotechnology, Amity University Uttar Pradesh Lucknow, India
2Ram Manohar Lohia Institute of Medical Sciences Lucknow, India
*Corresponding author

The nosocomial infections are most important medical complications in all areas of the world and they remain one of the main sources of morbidity and mortality in hospitalized patients. Acinetobacter bacteria has appeared over the last era as significant opportunistic pathogen liable for nosocomial infections, although it is commonly related with benign colonization of hospitalized patients. The present investigation is carried out to isolate, identify Acinetobacter species from the different samples collected from tertiary care hospitals. Further susceptibility and resistance of different antibiotics against Acinetobacter is performed in the present study. A total of 30 samples from various sources (wound pus, urine, respiratory secretion, blood) were collected from hospital unit. Isolation is done for different samples by using different media as blood agar and MacConkey agar for pus samples, Chrom and CLED for urine samples, Blood agar, MacConkey agar and Chocolate agar for respiratory secretions and isolation from blood samples is done by using automated blood culture system. Acinetobacter isolates were initially identified according to the morphological characteristics on MacConkey agar and further identification is performed on the basis of biochemical characterization. The isolates of Acinetobacter are identified as Non-fermenter, Gram-negative coccobacilli on the basis of the Catalase positive, Oxidase negative, Urease negative, Nitratenegative, Indole negative and motility test. Antibacterial susceptibility testing was performed by Kirby Bauer disc diffusion method allowing to CLSI guidelines. Most of the isolates were obtained from blood samples. It has showed very high resistance (>75%) to Imipenem, Ceftazidime, Ciprofloxacin, Tobramycin, Doripenem. Only some of isolates have showed to be effective agents against gentamycin, amikacin and ampicillin sulbactam.

Keywords: Acinetobacter species, Tertiary care hospital, Nosocomial infection, Antibiotic susceptibility, Biochemical identification, Disc diffusion method.

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Preeti Chandra, Vineeta Mittal and Rachna Chaturvedi. 2017. Isolation, Characterization and Antibacterial Susceptibility test of Acinetobacter Species Obtained from Tertiary Care Hospital.Int.J.Curr.Microbiol.App.Sci. 6(2): 1279-1286. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.