National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Neonatal sepsis is one of the leading causes of neonatal mortality in India. There has been a reduction in early-onset sepsis due to advances in obstetric care while incidence of late-onset has increased especially in very low birth weight infants. Diagnosis of neonatal sepsis remains difficult due to non-specific signs and symptoms in neonates and delay in diagnosis leads to complications. Although blood culture has been the gold standard in detection of bloodstream pathogens it lacks rapidity and sensitivity which are overcome by molecular techniques. In the present prospective study conducted from April 2017 to March 2018, a multiplex PCR assay was evaluated for detection of pathogen DNA directly from whole blood of neonates with late-onset sepsis in a tertiary care hospital. Blood culture was positive in 71/210 (33.81%) neonates with Coagulase negative Staphylococcus species being the most common isolate (28.38%). PCR added a statistically significant advantage in sensitivity (40.71% vs 37.14%) and negative predictive value over blood culture and also added a diagnostic yield by detecting additional 17 isolates that were missed by conventional blood culture. PCR and blood culture can identify a partially overlapping set of bloodstream infections making PCR a useful adjunct to blood culture.
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