'' '' '' '' Production of Cellulolytic Enzymes by Endophytic Fungi Isolated from Cameroonian Medicinal Plants
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here
ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here
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Original Research Articles                      Volume : 6, Issue:2, February, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(2): 1264-1271
DOI: http://dx.doi.org/10.20546/ijcmas.2017.602.142


Production of Cellulolytic Enzymes by Endophytic Fungi Isolated from Cameroonian Medicinal Plants
R.M.K. Toghueo1*, I.E. Ejiya2, D. Sahal3, S.S. Yazdani4 and F.F. Boyom1
1Antimicrobial and Biocontrol Agents Unit (AmBcAU), Laboratory for Phytobiochemistry and Medicinal Plants Studies, Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon
2Department of Microbiology, Olabisi Onabanjo University, Ago – Iwoye, Ogun State, Nigeria
3Malaria Research Laboratory, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India
4Microbial Engineering Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India
*Corresponding author
Abstract:

Cellulose constitutes bulk of the plant cell wall materials and the most abundant and renewable non-fossil carbon source on earth. Cellulose degrading fungi play an important role in recycling of cellulosic materials. Thus, this study was designed to screen endophytic fungi isolated from Cameroonian medicinal plants for their cellulase activity. The plate-clearing assay and enzyme assay methods were used for screening of potent cellulolytic endophytic fungi. The promising was chosen for filter paper cellulase (FPCase) and carboxymethyl cellulase (CMCase) assay. The most potent was Penicillium sp 51 (50mm), followed by P. chermesimum (30-37.5mm) and Penicillium sp (22.5-30mm). The total cellulase activity (FPase) was 0.36 U/mg and 0.44U/mg for avicel and CMC crude enzymes respectively. The optimal pH and temperature for activity of crude cellulases produced by Penicillium sp 51 were respectively from 5-6 and 40-50°C. This endophytic fungus can be used for industrial bioconversion of cellulose.


Keywords: Endophytic fungi, Cellulose, Filter paper cellulase, Carboxymethyl cellulose
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How to cite this article:

Toghueo, R.M.K., I.E. Ejiya, D. Sahal, S.S. Yazdani and Boyom, F.F. 2017. Production of Cellulolytic Enzymes by Endophytic Fungi Isolated from Cameroonian Medicinal Plants.Int.J.Curr.Microbiol.App.Sci. 6(2): 1264-1271. doi: http://dx.doi.org/10.20546/ijcmas.2017.602.142
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