National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Outer membrane proteins (OMPs) play a significant role in the pathogenesis of Pasteurellosis. Outer membrane proteins of P. multocida are reported to be immunogenic and protective. OMPs of Pasteurella multocida (P. multocida) B:2 vaccine strain P52 was characterized by using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) technique. The P. multocida P52 OMP demonstrated the presence of six OMP bands. The major bands were of 20, 34, 48, 72, 89 and 91 kDa. Potential immunogens of P. multocida P52 were identified by electroblot immunoassay. Immunoblotting was performed using the hyperimmune serum raised against the whole cell antigen. Polypeptides separated on 12% SDS-PAGE were transferred to nitrocellulose membrane by semi-dry system. Presence of three polypeptides of 34, 72 and 91 kD size was reported showing reaction with antiserum. A single band of 34kDa in SDS-PAGE was evident and was confirmed by western blot analysis.
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