National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study explores the bioconversion by Geotrichum candidum of five amino acids to their corresponding α-keto acids used in the treatment of chronic kidney disorder. The amino acid oxidase activity biotransforming amino acids to α-keto acids was detected in biomass as well as extracellular culture fluids of G. candidum. Bioconversion was determined by measuring α-keto acid production along with residual amino acid. There was a 2-fold increase in the amount of α-keto acids produced over a period of 24 hours by permeabilization of biomass when compared to immobilized biomass. Permeabilized G. Candidum biomass was further immobilized by entrapment in Na-alginate and studied for the production of α-keto acids from the corresponding amino acids. The permeabilized and immobilized biomass beads showed an additional 2-fold increase in the α–keto acids production as compared to only permeabilized cells. Decomposition of hydrogen peroxide formed in the enzymatic reaction by manganese oxide incorporated in sodium alginate beads further improved the yield. The bioconversion efficiency of the studied amino acids to their corresponding keto acids obtained in the presence of MnO2 by immobilized biomass of G. candidum at 4 h was found to be 29-34%.
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