International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:2, February, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(2): 955-960

Isolation, Serotyping and In-vitro and In-vivo Antibacterial Sensitivity of Escherichia coli Strains Isolated from Diarrheic Lambs in Kashmir
N.Hassan* and G.N. Sheikh
Department of Veterinary Medicine, Faculty of Veterinary Sciences,Sher-e-Kashmir University of Agricultural Sciences and Technology, Kashmir,Shuhama Campus Srinagar– 190 006, J&K, India
*Corresponding author

This study was aimed to isolate the Escherichia coli strains and to compare the in-vitro and in-vivo antibacterial sensitivity of Escherichia coli strains isolated from diarrheic lambs. Different strains of Escherichia coli were isolated from the clinically affected lambs. Principal serotypes of E. coli recovered were O20 (10.09 %), O11 (8.25%), O123, O84 and O22 (7.33% each), O107 (5.50%), O89 and O92 (3.66% each), O69 and O5 (2.75% each), O157, O15, O36, O159 and O60 (1.83% each). Serogroups O26, O23, O17, O157, O153 and O5 with established zoonotic importance were among those recovered from diarrhoeic lambs. The antimicrobial susceptibility pattern of the isolates showed isolates were sensitive to four antibacterials, viz. gentamicin (61.33%), ciprofloxacin (60%), neomycin (54.6%) and co- trimoxazole (50.6%). while as, the isolates were resistant to amoxicillin and oxacillin. The therapeutic efficacy (in-vivo) of four highly effective antibacterials were evaluated and treatment regimen used in four different groups of clinical cases indicated, gentamicin and ciprofloxacin most effective drugs followed by neomycin and co-trimoxazole which was comparable with their in-vitro efficacy.

Keywords: Diarrhea, Lambs, in-vitro, in-vivo, Escherichia coli, Serotypes, Antibacterial sensitivity.

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How to cite this article:

Hassan, N. and Sheikh, G.N. 2017. Isolation, Serotyping and In-vitro and In-vivo Antibacterial Sensitivity of Escherichia coli Strains Isolated from Diarrheic Lambs in Kashmir.Int.J.Curr.Microbiol.App.Sci. 6(2): 955-960. doi:
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