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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:10, October, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(10): 2009-2017
DOI: https://doi.org/10.20546/ijcmas.2019.810.234


In vitro Response of Promising Sugarcane Varieties for Salinity Tolerance through Callus Culture
Patel Devangi*, S.C. Mali2, J. Udutha2 and Patel Nilpa3
1Department of Genetics and Plant Breeding, 2Main Sugarcane Sugarcane Research, 3Department of Plant Molecular Biology and Biotechnology,Navsari Agricultural University, Navsari, India
*Corresponding author
Abstract:

Present investigation was carried out to screen two promising sugarcane varieties CoN 13073 and CoN 09072 for salinity tolerance through callus culture. Various physiological and morphological parameters was studied, maximum shoot length was observed at 2.0 % NaCl concentration treated to callus at 65 days of culturing in variety CoN 13073, similar trend was observed for root length and leaf number per plant in variety CoN 13073 at 2.0 % NaCl concentration.. Very little differences observed in shoot: root ratio in variety CoN 09072, while there was much difference was observed in shoot : root ratio at various levels of NaCl in variety CoN 13073S. Leaf area, chlorophyll content and Na:K ratio were decreased with increased the concentration of NaCl in both the varieties Shoot regenerated under high concentrations of NaCl shown maximum chlorophyll content and leaf area in both the varieties. In comparatives studies, CoN 13073 responded positively to higher concentrations for all the characters studied.


Keywords: Sugarcane, Salinity, Tissue culture, in vitro, Somaclonal variation

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How to cite this article:

Patel Devangi, V., S.C. Mali, J. Udutha and Patel Nilpa. 2019. In vitro Response of Promising Sugarcane Varieties for Salinity Tolerance through Callus Culture.Int.J.Curr.Microbiol.App.Sci. 8(10): 2009-2017. doi: https://doi.org/10.20546/ijcmas.2019.810.234
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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