National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The highly polymorphic gene of the MHC is central molecule in immune system in recognition of pathogens and parasites. The purpose of this study was to study the polymorphism of CLA-DRB3 gene in Salem black goat using PCR-RFLP technique. A region of exon 2 encompassing 285 bp fragment of DRB 3 gene was amplified by polymerase chain reaction. The restriction digestion by TaqI revealed three genotypes AA (285 bp), AB (285/163/122 bp) and BB (163/122 bp) with frequencies 0.220, 0.490 and 0.290, respectively and two alleles A and B with frequencies 0.465 and 0.535, respectively. The polymorphic information content (PIC) value and expected heterozygosity were 0.374 and 0.490, respectively which were low in both cases. The present study showed polymorphic nature of MHC Class II DRB3 Gene in Salem black goats at this locus and the frequencies of heterozygote were greater than homozygote.
Citations |
 |
 |
 |
 |
