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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:9, September, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(9): 2499-2505
DOI: https://doi.org/10.20546/ijcmas.2019.809.290


Evaluation of Local Trichoderma Isolates against Potential Soil Borne Pathogens of Pulses
A.G. Panda*, M. K. Mishra, S. K. Beura and B. Boblina
Department of Plant Pathology, College of Agriculture, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha-751003, India
*Corresponding author
Abstract:

Use of biocontrol agents against soil borne pathogen is gaining importance in the present situation for eco-friendly management of soil borne diseases. Bioefficacy of indigenous Trichoderma spp has greater efficacy against soil borne pathogens of pulses. Native Trichoderma isolates were collected from soil rhizospheres of paddy, groundnut, brinjal and tomato grown in the Agronomy field and Central farm of Orissa University of Agriculture and Technology, Bhubaneswar and named as Isolate 1, Isolate 2, Isolate 3, Isolate 4, Isolate 5 respectively. Another two isolates were collected from banana fruit (Bhubaneswar) and IIHR Bangalore (T.harzianum) and named as Isolate 6 and Isolate 7 (IIHR) respectively. Isolate 2 and Isolate 7 were found to sporulate vigorously and Isolate 5 was fast growing. Highest length of phialide (9.89 µm) was found in Isolate 2 and lowest in Isolate 7 (7.84 µm). Isolate 1 was having the widest phialide (4.82 µm) followed by Isolate 5 with the lowest breadth (2.99 µm). Isolate 7 produced the largest spore of 5.02 µm diameter and Isolate 1 with the lowest i.e 3.6 µm. Fusarium sp and Rhizoctonia sp were isolated from wilted green gram plants in the fields of OUAT farm. Isolate 5 inhibited 56.40 % radial growth of Rhizoctonia sp. followed by Isolate 2 (54.20 %), Isolate 5 reduced 70.1 % radial growth of Fusarium sp. followed by Isolate 2.Isolate 2 and 5, through soil application, enhanced 75.97% and 75.12% seedling length in comparison to control respectively against Rhizoctonia sp. and enhanced 75.55% and 84.08% seedling length against Fusarium sp. soil inoculation.


Keywords: Fusarium oxysporum, Crown rot, Aspergillus flavus

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How to cite this article:

Panda, A. G., M. K. Mishra, S. K. Beura and Boblina, B. 2019. Evaluation of Local Trichoderma Isolates against Potential Soil Borne Pathogens of Pulses.Int.J.Curr.Microbiol.App.Sci. 8(9): 2499-2505. doi: https://doi.org/10.20546/ijcmas.2019.809.290
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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