National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present investigation was conducted to optimize an efficient callus induction protocol in Carica papaya (cv. Pusa Nanha). Two different types of explants were collected from field grown papaya plants under study viz., leaf and petiole. The explants were surface sterilized and excised into 1-2 cm diameter and inoculated on basal MS medium supplemented with various concentrations of NAA, Kinetin and TDZ. The cultures were kept in dark inside the culture room at 25 ± 2°C and humidity 60-65%. The results obtained under study indicate that media containing only NAA and Kinetin results in less percent of callus induction in both explants (11.33 to 30% in leaf and 3.66 to 7% in petiole) whereas earliest and significantly (p<0.05) higher callus induction was obtained in media containing 3.0 mg/L NAA + 0.5 mg/L Kin and 1.5 µM TDZ. Callus produced from leaf was soft and spongy and white in colour while petiole produced hard and friable callus appeared dark in colour. The findings of study suggesting leaf as the best explant and TDZ as an effective growth regulator for callus production in papaya. This protocol for callus induction may also help in propagating papaya plants via somatic embryogenesis, organogenesis and transformation studies.
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