Preservation of boar semen at 18°C temperature requires climatic box which is costlier offence. Present study investigated seminal status at refrigeration (5°C) temperature in comparison to commonly used storage temperature (18° C). A total of 72 semen ejaculates were collected, diluted with KIEV extender and incubated at 2 different storage temperatures (18°C and 5°C) for 24, 48 and 72 hours, and seminal attributes were analyzed after each period. Percentage of motile sperm, live sperm, sperm with intact acrosome and HOSST positive sperms decreased significantly (p<0.01) as incubation intervals increases at both 18°C and 5°C storage temperature. However, these parameters showed a significantly (p<0.01) higher values at 18°C compared to 5°C. It can be concluded that semen of Large White Yorkshire boar extended with KIEV show better seminal attributes for short term storage at 18 °C in comparison to 5°C and this study provides further opportunities to explore the cause and effects for detrimental seminal attributes at 5°C.

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