International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2022 - IJCMAS--ICV 2022: 95.28 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020] For more details click here

Login as a Reviewer

See Guidelines to Authors
Current Issues
Download Publication Certificate

Original Research Articles                      Volume : 8, Issue:6, June, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(6): 3392-3402

An Efficient Protocol for in vitro Regeneration of Banana var. Nanjangudu rasabale (Musa spp. AAB)
P. Babu*
College of Agriculture, Hanumanamatti, UAS, Dharwad, India
*Corresponding author

Banana variety Nanjanagudu rasabale has been found growing in parts of Mysuru district, known for its unique aroma, flavour, taste and shelf life but unfortunately deemed as an endangered variety. Having given GI protection hope for bringing this variety back to field by producing disease free tissue culture plants demanded development of an efficient protocol for in vitro regeneration. In the present investigation, both citric acid and ascorbic acid found effective in inhibiting browning of shoot tip explant due to phenolic compounds. Each of the chemical sterilants was effective in reducing microbial contamination when they were used in sequence one after the other.MS media supplemented with BAP at 3.0 mg/l took least number of days for shoot regeneration and favoured better shoot production with maximum number of leaves per shoot and shoot length. Addition of 25mg/l adenine sulphate and 2-3 ml of Aonla juice proliferated maximum number of shoots during multiplication. MS media at half strength provided with activated charcoal and IBA at 2 mg/l was effective in producing better roots from in vitro grown shoots.

Keywords: Banana, Variety Nanjanagudu Rasabale, In vitro regeneration, BAP, IBA

Download this article as Download

How to cite this article:

Babu, P. 2019. An Efficient Protocol for in vitro Regeneration of Banana var. Nanjangudu rasabale (Musa spp. AAB).Int.J.Curr.Microbiol.App.Sci. 8(6): 3392-3402. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.