International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:6, June, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(6): 2931-2948

In vitro Study on the Efficacy of Silver Nanoparticles against Metallo-Beta-Lactamase and Biofilm Producing Pseudomonas aeruginosa Isolates
Marwa Gamal Bahey1*, Abou El Fetouh Eid El Enany1, Mohamed Ismaeil Abd El Hamid1, Aziza Mahmoud Hassan1 and Lobna Mohamed Abo Elnasr2
1Medical Microbiology & Immunology Department, Faculty of Medicine,Tanta University, Egypt
2Anesthesia and Surgical ICU Department, Faculty of Medicine,Tanta University, Egypt
*Corresponding author

Metallo beta-lactamase (MBL) and biofilm production is the most worrisome resistant mechanisms observed in Pseudomonas aeruginosa. Increased resistance of Pseudomonas to antibiotic therapy is an emerging global health concern which can be overcomed by Silver nanoparticles (AgNPs). Therefore this study aims to evaluate the antibacterial and antibiofilm activity of Silver nanoparticles on MBL and biofilm producing P. aeruginosa isolates using Transmission Electron microscope (TEM) and Confocal laser scanning microscope (CLSM) respectively. A total 40 isolates of p. aeruginosa were screened for MBL production by combined disc test. Then the isolates were screened for biofilm forming ability by tissue culture plate assay. Commercially available Silver nanoparticles were obtained and tested against MBL and biofilm producing isolates. The antibacterial activity of AgNps was tested by MIC and MBC methods and by TEM. The CLSM results exhibited AgNPs concentration dependent inhibition of bacterial growth and EPS matrix of the biofilm. Therefore, AgNps serve as the next generation antimicrobials in protection against the biofilm mediated infections. It is suggested that coating of medical devices with silver nanoparticles is recommended for treatment of resistant bacterial infections.

Keywords: Silver nanoparticles, Pseudomonas aeruginosa, Metallo Beta Lactamase, Biofilm, Transmission Electron Microscope, Confocal Laser Scanning Microscope

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How to cite this article:

Marwa Gamal Bahey, Abou El Fetouh Eid El Enany, Mohamed Ismaeil Abd El Hamid, Aziza Mahmoud Hassan and Lobna Mohamed Abo Elnasr. 2019. In vitro Study on the Efficacy of Silver Nanoparticles against Metallo-Beta-Lactamase and Biofilm Producing Pseudomonas aeruginosa Isolates.Int.J.Curr.Microbiol.App.Sci. 8(6): 2931-2948. doi:
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