National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Marssonina leaf blotch caused by the fungus Marssonina coronaria (Ellis & J.J. Davis) J.J. Davis causing premature leaf fall is one of the major economic constraint which hampers apple production in India. Seven isolates of Marssonina coronaria were isolated and evaluated for their morphological and molecular characterization using ITS primers. All the seven isolates were identified based on the morphological characteristics, and produced brown to black colonies without aerial mycelium, formed a wrinkled surface, on PDA after incubation for 30 days. Conidia are ampule shaped, one sepate, constricted at the septum, hyaline, and guttulate. However, not much difference was obtained in colony diameter and size of conidia. Seven isolates of the rDNA of ITS region was amplified by ITS4/ITS6 primers and ranged from 565~615 bp in the isolates. On BLAST, isolates showed high homologies (96~100%) with Marssonina coronaria. Phylogentic tree depicted that the isolates were closely clustered with Chinese isolates with accession numbers FJ606800, EU329733, EU329735, FJ618562, FJ618561 and FJ618560. The present study demonstrated the genetic relationships among the isolates of Marssonina coronaria and indicated that ITS rDNA sequence data were potentially useful in taxonomic species determination.
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