Genetic diversity using Random Amplified Polymorphic DNA (RAPD) markers was assessed in forty eight Indian potato germplasm. Twenty RAPD markers amplified a total of ninety-one different loci that exhibited 82.30 per cent polymorphism among 48 potato germplasm. The PIC value ranged from 0.27 to 0.55. All the loci amplified by the primer which were found to be polymorphic varied in size from <50bp to >1500bp. The Jaccard’s similarity coefficient was found to vary from 0.22 to 0.87. The maximum genetic similarity (0.87) was found between Kufri Badshah and Kufri Khyati and lowest (0.22) between Pant Sel.-01-15 and Pant Sel.-09-57. UPGMA based dendrogram ordered the populations of genotypes into nine clusters. The most diverse groups found were Cluster II and cluster IX followed by cluster I and VII with Cluster II. RAPD markers were found to be sufficiently sensitive to detect genetic variation among various germplasm, which will be useful for selecting genetically distinct germplasm in potato breeding programme.

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