International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:6, June, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(6): 2391-2398

Studies on in vitro Culture of Coconut var. Chowghat Orange Dwarf through Direct Organogenesis
D.S. Sushmitha, R. Renuka* and D. Chandrakala
Plant Tissue Culture Laboratory, Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore – 641003, Tamil Nadu, India
*Corresponding author

Coconut has a wide range of applications from culinary to industrial areas. Loss of coconut trees due to the devastating effect of deadly diseases and the natural aging of the plants demand rejuvenation of orchards with elite seedlings. A conventional breeding program is not sufficient to meet the growing need for healthy planting materials. However, in vitro culture studies can pave the way for mass multiplication. In this study, the effect of TDZ on sliced mature embryo explants through direct organogenesis of coconut var. Chowghat Orange Dwarf (COD) was investigated. The treatment Y3 + 150µM TDZ recorded highest shoot induction (90.91 %) and shoot regeneration (72.73 %). A higher concentration of TDZ reduced the frequency of shoot organogenesis. Well-grown shoots were transferred to rooting media and plantlets were subjected to primary hardening.

Keywords: Coconut, Direct organogenesis, TDZ, Sliced mature embryo, Shoot regeneration, Chowghat Oragne Dwarf

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How to cite this article:

Sushmitha, D.S., R. Renuka and Chandrakala, D. 2019. Studies on in vitro Culture of Coconut var. Chowghat Orange Dwarf through Direct Organogenesis.Int.J.Curr.Microbiol.App.Sci. 8(6): 2391-2398. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.