Survey was conducted in grapevine growing areas of Coimbatore district during October to December 2018 to assess the incidence of powdery mildew and to collect different isolates of Ampelomyces spp. The powdery mildew incidence was noticed from 14.65 to 63.21 PDI. Ten isolates of Ampelomyces spp. were collected from powdery mildew infected grapevine leaf parasitized by Ampelomyces spp. All the isolates were identified by their morphological characters. Most of the isolates showed flat and radial growth pattern with raised growth of mycelia brownish black to greyish white. The pycnidia of different isolates of Ampelomyces varied in their shape and were mostly ovoid, ellipsoid, cylindrical, spindle, pyriform to globose in shape. Ampelomyces spp. was studied for its morphological characters in the present work.

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