International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:6, June, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(6): 1676-1683

Morphological and Ultrastructure of Developmental Stages of Somatic Embryos of Popular Banana Cultivars
Ruma Debbarma1, D. Sudhakar2, K.K. Kumar2 and K. Soorianathasundaram1*
1Department of Fruit Science, HC & RI, TNAU, Coimbatore-03, Tamilnadu, India
2Department of Plant Biotechnology, CPMB & B, TNAU, Coimbatore-03, Tamilnadu, India
*Corresponding author

Somatic embryogenesis based plant regeneration is a pre-requisite for genetic transformation of banana. Mass multiplication of banana through somatic embryogenesis is also viewed as an economically viable alternative to commonly used shoot tip based method. Banana somatic embryogenesis response was studied in three popular banana cultivars, Grand Naine, Rasthali and Hill banana ‘Virupakshi’. Immature male flower bud explant was used for embryogenic callus induction. Different morphological types of calli were produced during callus induction that includes both embryogenic and non-embryogenic calli. White translucent pro-embryogenic calli were found to be suitable for establishment of embryogenic cell suspension (ECS). Regeneration potential of ECS was assessed and the development of different stages of embryo viz., globular, scutellum, and coleoptillar stages was observed under scanning electron microscope to understand their ultra-structure. The differences in the duration of developmental stages and regeneration potential could be attributed to the genomic differences of cultivars involved.

Keywords: Somatic embryogenesis, embryogenic calli, non-embryogenic calli, cell suspension cultures, somatic embryo, scanning electron microscope

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How to cite this article:

Ruma Debbarma, D. Sudhakar, K.K. Kumar and Soorianathasundaram, K. 2019. Morphological and Ultrastructure of Developmental Stages of Somatic Embryos of Popular Banana Cultivars.Int.J.Curr.Microbiol.App.Sci. 8(6): 1676-1683. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.