National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Somatic embryogenesis based plant regeneration is a pre-requisite for genetic transformation of banana. Mass multiplication of banana through somatic embryogenesis is also viewed as an economically viable alternative to commonly used shoot tip based method. Banana somatic embryogenesis response was studied in three popular banana cultivars, Grand Naine, Rasthali and Hill banana ‘Virupakshi’. Immature male flower bud explant was used for embryogenic callus induction. Different morphological types of calli were produced during callus induction that includes both embryogenic and non-embryogenic calli. White translucent pro-embryogenic calli were found to be suitable for establishment of embryogenic cell suspension (ECS). Regeneration potential of ECS was assessed and the development of different stages of embryo viz., globular, scutellum, and coleoptillar stages was observed under scanning electron microscope to understand their ultra-structure. The differences in the duration of developmental stages and regeneration potential could be attributed to the genomic differences of cultivars involved.
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