International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:6, June, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(6): 1453-1458

Utility of Seed Germination Inhibition Test for Early Pregnancy Diagnosis in Buffaloes
V. Aswathnarayanappa, P.M. Gururaj*, Nagappa Banuvalli and M. Harisha
Department of Veterinary Physiology and Biochemistry, Veterinary College, Shivamogga-577204, Karnataka, India
*Corresponding author

Early pregnancy diagnosis plays a key role for profitable and sustainable animal husbandry practices especially in the productive animal species like cattle and buffaloes. In this context, a study was undertaken to assess the utility of seed germination inhibition technique (Punyakoti test) for early detection of pregnancy in buffaloes. Thirty non-descript buffaloes were randomly selected from the different villages of Bangalore Rural district. The urine samples from selected buffaloes were collected while they were naturally micturating early in the morning on day 30, 45 and 60 after artificial insemination and the samples were subjected to seed germination inhibition test within four to six hours of collection. Wheat, green gram and ragi seeds were subjected for germination after diluting the urine sample with distilled water in the ratio of 1:4. The per cent seed germination after 48 hours and the extent of shoot length on the fifth day were recorded. The pregnancy status of each test animal was confirmed per rectally on day 60 after artificial insemination. There was no significant difference (P>0.05) in seed germination inhibition per cent on day 30 between inseminated buffaloes and confirmed non pregnant buffaloes. The germination inhibition per cent of the seeds of wheat, green gram and ragi among inseminated pregnant, inseminated non pregnant, confirmed non pregnant and distilled water control group differed significantly (P < 0.05) on day 45 and 60. There was no significant difference (P>0.05) in shoot length on day 30 between pregnant and non-pregnant buffaloes. However, the shoot length of all the seeds of wheat, green gram and ragi differed significantly (P < 0.05) in inseminated pregnant buffaloes compared to inseminated non pregnant, confirmed non pregnant and distilled control group on day 45 and 60. Thus, from the present study, it was concluded that the seed germination inhibition test could be performed to detect early pregnancy in buffaloes.

Keywords: Early pregnancy, Seed germination inhibition

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How to cite this article:

Aswathnarayanappa, V., P.M. Gururaj, Nagappa Banuvalli and Harisha, M. 2019. Utility of Seed Germination Inhibition Test for Early Pregnancy Diagnosis in Buffaloes.Int.J.Curr.Microbiol.App.Sci. 8(6): 1453-1458. doi:
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