National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was focused on the biodegradation of pine needles by the production of hydrolytic enzymes viz. cellulase, xylanase, laccase as well as reducing sugars production w.r.t optimization of moisture (Basal salt media) ratio for the newly isolated white rot fungi Phanerochaete sp. S7, Armillaria sp. K3 and Phanerochaete chrysosporium [NCIM 1197] were optimized under solid state fermentation and maximum of cellulase (52.27 U/g) with specific activity of 2.10 was observed in P. chrysosporium using untreated pine needles as substrate at 1:4 of moisture ratio, while maximum of xylanase and laccase were expressed by white rot Armillaria sp K3 at 1:4 of moistening agent i.e.174.00 U/g and 5.94 U/g with respect to their specific activities of 7.65 and 0.28 using untreated pine needles. In case of reducing sugars maximum of 15.48 mg/g was released at 1:4 of moisture using pretreated pine needles which is followed by the value of 14.88 mg/g at 1:3 by P. chrysosporium in untreated pine needles. In our study the newly isolated white rot fungi Phanerochaete sp. S7 and Armillaria sp. K3 were found to be potential strains for the hyper production of lignin degrading enzyme i.e. laccase as compared to the standard strain Phanerochaete chrysosporium [NCIM 1197].
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