International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:6, June, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(6): 511-517

Detection and Molecular Characterization of Black Spot Disease of Papaya (Carica papaya L.) Incited byAsperisporium caricae (Speg.) Maubl
S. Shreedevasena1*, S.K. Manoranjitham1, L. Rajendran1 and R. Parimaladevi2
1Department of Plant Pathology,
2Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore -641 003, Tamil Nadu, India
*Corresponding author

Black spot of papaya caused by Asperisporium caricae incidence was recorded in five different localities viz., Pasur, Telungupalayam, Thondamuthur, Vedapatti and TNAU orchard of Coimbatore district. Per cent disease severity (PDS) ranged from 1 to 29 per cent on leaves. The fungal pathogen associated with black spot of papaya was isolated and identified as Asperisporium caricae based on morphological characters. Severe disease incidence was observed from November to January and no disease incidence was recorded during summer. Pathogenicity test was proved on young papaya plants under glasshouse conditions twenty days after inoculation. Molecular confirmation of Asperisporium caricae through 18S rRNA gene sequencing gave an amplicon size of 560bp size. The isolate Asperisporium caricae was sequenced and submitted to NCBI database with the accession number MK879405. The present study was aimed to characterize the pathogen responsible for black spot of papaya and confirmation by molecular methods.

Keywords: Papaya, black spot, Molecular detection, Asperisporium caricae

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How to cite this article:

Shreedevasena, S., S.K. Manoranjitham, L. Rajendran and Parimaladevi, R. 2019. Detection and Molecular Characterization of Black Spot Disease of Papaya (Carica papaya L.) Incited byAsperisporium caricae (Speg.) Maubl.Int.J.Curr.Microbiol.App.Sci. 8(6): 511-517. doi:
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