National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Pseudomonas aeruginosa is considered as the epitome of an opportunistic pathogens of hospitalized patients. A total of 159 clinical specimens were recovered and molecular identified by PCR assay. The number of isolates which identified as P. aeruginosa was 101 clinical isolates. Considering the effect of 14 natural products/extracts on some virulence factors of MDR isolates, sub inhibitory concentration (½ MIC) of Syzygium aromaticum (clove) buds extract inhibit the activity of alkaline protease enzyme on skimmed milk agar. The swarming motility was decreased while phospholipase C enzyme activity was increased by sub inhibitory conc. of clove buds extract. Biofilm formation of P. aeruginosa was inhibited by Psidium guajava (guava) leaves extract at ½ MIC (showed pink colonies on congo red agar).The effect of different conc. of Psidium guajava leaves extract on outer membrane proteins of Pseudomonas aeruginosa isolate was determined by SDS-PAGE and analyzed using Image J program. Sub inhibitory conc. (¾ MIC) of guava leaves extract showed lower relative density in protein bands at molecular weights of 70 KDa, 65 KDa , 60 KDa , 59 KDa , 49 KDa ,44 KDa& 36 KDa compared to the control.
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